3JV3
Structure of SH3E-DH unit of murine intersectin-1L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 67.044, 67.044, 341.818 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.260 - 2.400 |
| R-factor | 0.25 |
| Rwork | 0.248 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.387 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.050 | 0.024 | 0.386 |
| Number of reflections | 36102 | ||
| <I/σ(I)> | 38.764 | ||
| Completeness [%] | 99.5 | 98.4 | 97.1 |
| Redundancy | 7.6 | 7.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 1.7 M LiSO4, 0.1M Hepes pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
