3JUU
Crystal structure of porphyranase B (PorB) from Zobellia galactanivorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.131, 120.892, 67.919 |
| Unit cell angles | 90.00, 101.50, 90.00 |
Refinement procedure
| Resolution | 44.700 - 1.800 |
| R-factor | 0.15924 |
| Rwork | 0.157 |
| R-free | 0.19856 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ilf |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.217 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER ((CCP4)) |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.200 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.089 | 0.395 |
| Number of reflections | 62907 | |
| <I/σ(I)> | 11 | 3.7 |
| Completeness [%] | 100 | |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 33-35% PEG 1000, 0.4M Lithiumsulfate, 0.1M Mes pH 6.0, 10% Glycero, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
