3JUD
Human gamma-glutamylamine cyclotransferase, E82Q mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.826966 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.307, 42.443, 84.764 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.570 - 0.980 |
| R-factor | 0.15478 |
| Rwork | 0.153 |
| R-free | 0.17969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vkb |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.313 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.950 | 1.030 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rmerge | 0.104 | 0.688 |
| Number of reflections | 74200 | |
| <I/σ(I)> | 10.4 | 2.5 |
| Completeness [%] | 97.7 | 100 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 12.5%(w/v) PEG3350, 0.3M ammonium nitrate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
