3JUC
Human gamma-glutamylamine cyclotransferase complex with 5-oxoproline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-03 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.954448 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.247, 42.478, 84.814 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.410 - 1.200 |
| R-factor | 0.1187 |
| Rwork | 0.117 |
| R-free | 0.14757 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vkb |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.922 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.520 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.055 | 0.154 |
| Number of reflections | 40928 | |
| <I/σ(I)> | 27.5 | 10.7 |
| Completeness [%] | 98.5 | 97 |
| Redundancy | 9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 22.5%(w/v) PEG3350, 0.2M ammonium nitrate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
