3JU8
Crystal Structure of Succinylglutamic Semialdehyde Dehydrogenase from Pseudomonas aeruginosa.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9788 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.117, 48.033, 187.130 |
| Unit cell angles | 90.00, 107.43, 90.00 |
Refinement procedure
| Resolution | 32.775 - 1.820 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.19600 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.464 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.4_138)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.780 | 1.850 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 104456 | |
| <I/σ(I)> | 12.5 | 2.9 |
| Completeness [%] | 99.3 | 91.9 |
| Redundancy | 4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1.6 M Magnesium sulfate 0.1 M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
