3JU5
Crystal Structure of Dimeric Arginine Kinase at 1.75-A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.881, 59.868, 139.873 |
| Unit cell angles | 90.00, 92.72, 90.00 |
Refinement procedure
| Resolution | 75.340 - 1.750 |
| R-factor | 0.17282 |
| Rwork | 0.170 |
| R-free | 0.22671 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.989 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.700 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.072 | 0.497 |
| Number of reflections | 132173 | |
| <I/σ(I)> | 20.6 | 1.68 |
| Completeness [%] | 98.2 | 85.2 |
| Redundancy | 3.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 0.1M Bis-Tris (pH 6.6), 12% PEG 1000, VAPOR DIFFUSION, HANGING DROP |
