3JU4
Crystal Structure Analysis of EndosialidaseNF at 0.98 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | H 3 |
| Unit cell lengths | 119.040, 119.040, 175.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 0.980 |
| R-factor | 0.1162 |
| Rwork | 0.116 |
| R-free | 0.13340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v0e |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.080 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Number of reflections | 516832 | |
| <I/σ(I)> | 12.41 | 3.42 |
| Completeness [%] | 96.9 | 99.6 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.2 | 293 | 16% (w/v) PEG 8000, 0.1M Tris/HCl pH 7.2, 3% Isopropanol, VAPOR DIFFUSION, temperature 293K |
