3JTY
Crystal structure of a BenF-like porin from Pseudomonas fluorescens Pf-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-31 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.103, 210.624, 84.066 |
| Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
| Resolution | 35.110 - 2.580 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Poly-Alanine model of the PDB code 2QTK |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.1.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.680 | 2.720 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Number of reflections | 67053 | |
| <I/σ(I)> | 6.1 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 100mM Citric acid, 21% PEG 3350, 10% w/v ANAPOE-X-114 from the detergent screen, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
