3JTT

Cystal structure of Rhesus macaque MHC class I:Mamu-A*02

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-05-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5478
Spacegroup name	P 21 21 21
Unit cell lengths	128.993, 129.007, 129.026
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.293 - 2.800
R-factor	0.2158
Rwork	0.214
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zvs
RMSD bond length	0.002
RMSD bond angle	0.660
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.074	0.528
Number of reflections	51015
<I/σ(I)>	37.2	4.7
Completeness [%]	99.6	100
Redundancy	7.1	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.1 M Bis-Tris pH 5.5, 2 M ammonium sulfate , VAPOR DIFFUSION, HANGING DROP, temperature 291K