3JTO
Crystal structure of the c-terminal domain of YpbH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 162.056, 162.056, 142.848 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.197 - 2.400 |
R-factor | 0.2324 |
Rwork | 0.230 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jtn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.089 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.084 | 0.782 |
Number of reflections | 37149 | |
<I/σ(I)> | 25.3 | 3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 8.1 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 2% PEG 400, 1.85M ammonium sulfate, 0.1M Hepes pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |