3JTN
Crystal Structure of the c-terminal domain of YpbH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-22 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.930, 71.660, 39.330 |
| Unit cell angles | 90.00, 95.35, 90.00 |
Refinement procedure
| Resolution | 34.363 - 2.090 |
| R-factor | 0.2219 |
| Rwork | 0.217 |
| R-free | 0.30350 |
| Structure solution method | PHASER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.084 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.363 | 2.210 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.079 | 0.732 |
| Number of reflections | 17571 | |
| <I/σ(I)> | 9.09 | 2.6 |
| Completeness [%] | 94.1 | 88 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 20% PEG 3350,0.3M calcium chloride, 0.1M Bis-Tris pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
