3JT1
Legionella pneumophila glucosyltransferase Lgt1, UDP-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Detector technology | PIXEL |
| Collection date | 2009-08-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 122.571, 122.571, 103.982 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.760 - 2.300 |
| R-factor | 0.18903 |
| Rwork | 0.185 |
| R-free | 0.26158 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jsz |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.949 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.800 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.102 | 0.657 |
| Number of reflections | 26541 | |
| <I/σ(I)> | 8.4 | 2.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 293.15 | 1ul protein (4mg/ml) was mixed with 1ul of reservoir solution containing 23% PEG3350, 0.06M ammonium acetat, 0.1M sodium acetate buffer, pH5.0, temperature 293.15K |
