3JSZ
Legionella pneumophila glucosyltransferase Lgt1 N293A with UDP-Glc
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Detector technology | PIXEL |
| Collection date | 2009-06-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00, 0.97973, 0.97957, 0.97205 |
| Spacegroup name | H 3 |
| Unit cell lengths | 122.232, 122.232, 102.779 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.960 - 1.700 |
| R-factor | 0.16701 |
| Rwork | 0.166 |
| R-free | 0.19318 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.182 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.062 | 0.391 |
| Number of reflections | 62915 | |
| <I/σ(I)> | 7.3 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 293.15 | 23% PEG3350, 0.06M ammonium acetat, 0.1M sodium acetate buffer, pH5.0, temperature 293.15K |
