3JSJ

Crystal structure of a putative tetr-transcriptional regulator (sav143) from streptomyces avermitilis ma-4680 at 2.10 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-17
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	56.401, 84.351, 103.390
Unit cell angles	90.00, 102.31, 90.00

Refinement procedure

Resolution	29.748 - 2.100
R-factor	0.191
Rwork	0.189
R-free	0.23000
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.327
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.748	29.748	2.170
High resolution limit [Å]	2.100	4.510	2.100
Rmerge	0.069	0.026	0.585
Number of reflections	55159	10751	10025
<I/σ(I)>	8.74	25.1	1.4
Completeness [%]	98.6	97.7	98.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.3000M magnesium chloride, 16.0000% polyethylene glycol 8000, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K