3JS8
Solvent-stable cholesterol oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.227, 90.380, 124.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.450 - 1.540 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i19 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.470 |
| High resolution limit [Å] | 1.540 |
| Number of reflections | 96731 |
| <I/σ(I)> | 22.51 |
| Completeness [%] | 93.3 |
| Redundancy | 3.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 500 mM ammonium sulfate, 1.0 M lithium sulfate, 10 mM sodium citrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
