3JRU

Crystal structure of Leucyl Aminopeptidase (pepA) from Xoo0834,Xanthomonas oryzae pv. oryzae KACC10331

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-03
Detector	ADSC QUANTUM 210
Wavelength(s)	0.96418
Spacegroup name	P 21 3
Unit cell lengths	152.126, 152.126, 152.126
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.020 - 2.600
R-factor	0.14828
Rwork	0.146
R-free	0.21531
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gyt
RMSD bond length	0.017
RMSD bond angle	1.674
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.020	2.640
High resolution limit [Å]	2.600	2.590
Number of reflections	35407
Completeness [%]	100.0	99.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	287	0.05M CaCl2, 0.1M Bis-Tris, 30% PEG monomethyl ether 550, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 287K