3JRT
Structure from the mobile metagenome of V. paracholerae: Integron cassette protein Vpc_cass2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 64 |
| Unit cell lengths | 85.510, 85.510, 46.656 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.990 - 2.300 |
| R-factor | 0.193 |
| Rwork | 0.189 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.968 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.4_151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.003 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.473 |
| Total number of observations | 18536 | |
| Number of reflections | 8801 | |
| <I/σ(I)> | 21.6 | 1.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.1 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 294 | 20% PEG3350, 0.2 M disodium tartrate. In situ proteolysis with chymotrypsin, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
