3JRO
NUP84-NUP145C-SEC13 edge element of the NPC lattice
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 170.472, 170.472, 270.732 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.887 - 4.004 |
| R-factor | 0.2858 |
| Rwork | 0.282 |
| R-free | 0.32890 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.762 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 37016 | |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 295 | 1.15M sodium malonate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
