3JR7
The crystal structure of the protein of DegV family COG1307 with unknown function from Ruminococcus gnavus ATCC 29149
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-04 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.899, 96.899, 159.921 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.920 - 2.000 |
| R-factor | 0.16432 |
| Rwork | 0.162 |
| R-free | 0.20843 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.673 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.920 | 2.055 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.352 |
| Number of reflections | 55821 | |
| <I/σ(I)> | 17.85 | 2.1 |
| Completeness [%] | 99.4 | 99.52 |
| Redundancy | 5.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | 0.2M Sodium chloride, 0.1M Phosphate-citrate, 10%PEG3000, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
