3JR3

Sir2 bound to acetylated peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Detector technology	CCD
Collection date	2007-03-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95
Spacegroup name	P 21 21 21
Unit cell lengths	45.717, 58.382, 106.516
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.550 - 1.500
R-factor	0.20578
Rwork	0.205
R-free	0.22787
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h2d
RMSD bond length	0.009
RMSD bond angle	1.203
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	22.550
High resolution limit [Å]	1.500
Rmerge	0.110
Number of reflections	43439
<I/σ(I)>	4.32
Completeness [%]	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.2	298	pH 9.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K