3JQR
Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Detector technology | CCD |
| Collection date | 2008-04-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.109, 96.689, 46.850 |
| Unit cell angles | 90.00, 118.00, 90.00 |
Refinement procedure
| Resolution | 37.290 - 2.300 |
| R-factor | 0.23022 |
| Rwork | 0.227 |
| R-free | 0.29028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ok8 chain A |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.882 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.145 | 0.710 |
| Number of reflections | 14346 | |
| <I/σ(I)> | 10.9 | 2.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch with permeable oil | 6.5 | 293 | 1.6M citrate-NaOH, pH 6.5, Microbatch with permeable oil, temperature 293K |
