3JQP
Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum with 2'P-AMP
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 |
| Unit cell lengths | 122.570, 122.570, 133.744 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.580 - 3.000 |
| R-factor | 0.24776 |
| Rwork | 0.244 |
| R-free | 0.31661 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ok7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.332 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.580 | 3.160 |
| High resolution limit [Å] | 2.800 | 3.000 |
| Rmerge | 0.157 | 0.775 |
| Number of reflections | 44994 | |
| <I/σ(I)> | 9.5 | 1.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch, permeable oil | 6.5 | 293 | 12% PEG 6000, 5% glycerol, 0.1 M cacodylate-NaOH , pH 6.5, Microbatch, permeable oil, temperature 293K |
