3IXS
Ring1B C-terminal domain/RYBP C-terminal domain Complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.9790, 0.9795, 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.894, 56.549, 100.352 |
| Unit cell angles | 100.88, 90.61, 100.49 |
Refinement procedure
| Resolution | 40.676 - 1.700 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.23390 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.060 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.4_4)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.680 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 84818 | |
| <I/σ(I)> | 7.3 | 1.9 |
| Completeness [%] | 95.9 | 83 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 32% PEG 1750, 0.125 M lithium sulfate, 10% ethylene glycol, 50 mM CHES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
