3IX7
Crystal structure of a domain of functionally unknown protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 61 |
| Unit cell lengths | 110.152, 110.152, 39.205 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.21167 |
| Rwork | 0.209 |
| R-free | 0.25609 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.431 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.170 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.049 | 0.427 |
| Number of reflections | 15031 | |
| <I/σ(I)> | 58.18 | 6.22 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.2 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2M Calcium acetate, 0.1M imidazol, 10% PEG8000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
