3IVP
The structure of a possible transposon-related DNA-binding protein from Clostridium difficile 630.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937, 0.97953 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.326, 69.829, 170.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.400 - 2.020 |
| R-factor | 0.20152 |
| Rwork | 0.200 |
| R-free | 0.23498 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.505 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.050 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.088 | 0.753 |
| Number of reflections | 39518 | |
| <I/σ(I)> | 25.8 | 1.8 |
| Completeness [%] | 99.5 | 94.7 |
| Redundancy | 7.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2M Ca Acetate, 0.1M Sodium Cacodylate, 40% v/w PEG300, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
