3IVN
Structure of the U65C mutant A-riboswitch aptamer from the Bacillus subtilis pbuE operon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91924 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.930, 46.330, 87.240 |
| Unit cell angles | 90.00, 120.04, 90.00 |
Refinement procedure
| Resolution | 26.039 - 2.800 |
| R-factor | 0.232 |
| Rwork | 0.227 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB id 1Y26 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.147 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.4_125)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.040 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 10839 | |
| <I/σ(I)> | 10.5 | 3.1 |
| Completeness [%] | 99.1 | 99 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 2 mM Spermine, 50 mM Na Cacodylate, 32% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
