3IV6
Crystal Structure of Putative Zn-dependent Alcohol Dehydrogenases from Rhodobacter sphaeroides.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 179.883, 179.883, 83.826 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.769 - 2.700 |
R-factor | 0.179 |
Rwork | 0.176 |
R-free | 0.23800 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.468 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.4_147)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.770 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 38199 | |
<I/σ(I)> | 6.3 | 3.3 |
Completeness [%] | 99.1 | 100 |
Redundancy | 9 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M HEPES, 25 % w/v PEG 3550, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |