3IV6
Crystal Structure of Putative Zn-dependent Alcohol Dehydrogenases from Rhodobacter sphaeroides.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 179.883, 179.883, 83.826 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.769 - 2.700 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.468 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.4_147)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.770 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 38199 | |
| <I/σ(I)> | 6.3 | 3.3 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 9 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M HEPES, 25 % w/v PEG 3550, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
