3IUZ

Crystal structure of Putative glyoxalase superfamily protein (YP_299723.1) from RALSTONIA EUTROPHA JMP134 at 1.90 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97860,0.97806
Spacegroup name	I 4 2 2
Unit cell lengths	114.069, 114.069, 133.518
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.904 - 1.900
R-factor	0.169
Rwork	0.167
R-free	0.20600
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.562
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.904	28.910	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.137	0.048	0.986
Total number of observations		2844	18804
Number of reflections	34930
<I/σ(I)>	11.9	11.2	0.8
Completeness [%]	100.0	97.6	100
Redundancy	7.4	6.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	40.0000% polyethylene glycol 600, 0.1M sodium citrate pH 5.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K