3IUV
The structure of a member of TetR family (SCO1917) from Streptomyces coelicolor A3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948, 0.97959 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.577, 58.577, 109.226 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.000 - 2.554 |
| R-factor | 0.2431 |
| Rwork | 0.241 |
| R-free | 0.28820 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.348 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.610 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.045 | 0.565 |
| Number of reflections | 7324 | |
| <I/σ(I)> | 59.6 | 2.7 |
| Completeness [%] | 97.9 | 85.5 |
| Redundancy | 10.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | 0.1M Na(CAc), 3.5M Naformate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
