3IUS
The structure of a functionally unknown conserved protein from Silicibacter pomeroyi DSS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-09 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.736, 81.648, 58.655 |
| Unit cell angles | 90.00, 94.65, 90.00 |
Refinement procedure
| Resolution | 39.440 - 1.660 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.923 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.097 | 0.609 |
| Number of reflections | 62442 | |
| <I/σ(I)> | 21.4 | 1.77 |
| Completeness [%] | 98.0 | 85.8 |
| Redundancy | 4.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M MgCl2, 0.1M Tris Hydrochloride, 25% w/v PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
