3IUL
apPEP_WT1 opened state
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-20 |
| Wavelength(s) | 0.97177,0.97934,0.97945 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.237, 92.066, 152.276 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.590 - 1.950 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.21800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 64722 | |
| <I/σ(I)> | 21.2 | 5.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 287 | 8mg/ml protein in 20mM Hepes (pH7.5), 100mM NaCl, 5% w/v glycerol, 1mM EDTA, and 1mM DTT. Equal volume of protein and precipitant (20mM MES (pH 6.5), 12% w/v PEG20K) were equilibrated by vapor diffusion at 14C. Cryosolution is precipitant plus 5% more PEG20K and 20% w/v glycerol., VAPOR DIFFUSION, temperature 287K |
