3IU7
M. tuberculosis methionine aminopeptidase with Mn inhibitor A02
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Collection date | 2008-11-05 |
| Wavelength(s) | 1 |
| Spacegroup name | P 63 |
| Unit cell lengths | 106.412, 106.412, 50.422 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.140 - 1.400 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yj3 |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.516 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 3.800 | 1.400 |
| Rmerge | 0.045 | 0.024 | 0.215 |
| Number of reflections | 64180 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 99.9 | 98.8 | 100 |
| Redundancy | 10 | 10.6 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Bis-Tris, pH 5.5, 1.1 M AMS, 50 mM NaCl, vapor diffusion, hanging drop, temperature 298K |
