3IU4
anti NeuGcGM3 ganglioside chimeric antibody chP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-10-08 |
| Detector | ADSC Q210 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 153.450, 153.450, 111.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.570 - 1.750 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1CEL |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.247 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.700 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.066 | 0.515 |
| Number of reflections | 63026 | |
| <I/σ(I)> | 19.2 | 4.2 |
| Completeness [%] | 99.4 | 14.5 |
| Redundancy | 8.8 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | Final data were collected on a crystal obtained from crystallization conditions containing 30% polyethylene glycol (PEG) 10K, 0.1 M cacodylate pH 6.5 and 0.2 M ammonium sulfate, which had grown to a size of 0.2x0.1x0.1 mm3 over 7.5 months. 15% glycerol was used as a cryo-protectant, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
