3IU0
Structural basis for zymogen activation and substrate binding of transglutaminase from Streptomyces mobaraense
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 180 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.333, 67.123, 83.969 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.129 - 1.900 |
| R-factor | 0.21319 |
| Rwork | 0.210 |
| R-free | 0.24394 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.056 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | 0.238 |
| Number of reflections | 29327 | |
| <I/σ(I)> | 39.2 | 8.1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 8.2 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 30% PEG8000, 50mM NaCl, 1mM EDTA, 1mM beta-mercaptoethanol, 0.01% NaN3, 100mM cacodylic acid, pH5.0 and 2% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
