3ISX
Crystal structure of Endoglucanase (TM1050) from THERMOTOGA MARITIMA at 1.40 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-13 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.383, 45.225, 66.787 |
| Unit cell angles | 90.00, 106.61, 90.00 |
Refinement procedure
| Resolution | 28.421 - 1.400 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.18100 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.719 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.421 | 28.421 | 1.450 |
| High resolution limit [Å] | 1.400 | 3.010 | 1.400 |
| Rmerge | 0.038 | 0.022 | 0.467 |
| Number of reflections | 64412 | 12281 | 12364 |
| <I/σ(I)> | 12.12 | 32.3 | 1.9 |
| Completeness [%] | 98.6 | 96.6 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 20.00% polyethylene glycol 3000, 0.1M citric acid pH 5.5, Additive: 0.001 M 1,10-phenanthroline, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 277K |
