3IST
Crystal structure of glutamate racemase from Listeria monocytogenes in complex with succinic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.420, 66.420, 258.774 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.17307 |
| Rwork | 0.171 |
| R-free | 0.21145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hfr |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.677 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 (MOLREP) |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.073 | 0.671 |
| Number of reflections | 69724 | |
| <I/σ(I)> | 39.4 | 2.4 |
| Completeness [%] | 97.9 | 92.6 |
| Redundancy | 6.5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG3350 15%, Succinic acid 0.1M, pH 7, 10mm L-Glutamic acid, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
