3IR8
Red fluorescent protein mKeima at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.959, 75.686, 85.674 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.180 - 1.630 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mov |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.414 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.059 | 0.505 |
| Number of reflections | 53662 | |
| <I/σ(I)> | 12.4 | 2.6 |
| Completeness [%] | 97.6 | 95.3 |
| Redundancy | 6.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 26% PEG 3350, 0.2 M NaCl, 0.1 M Tris pH 7.0, vapor diffusion, hanging drop, temperature 295K |
