3IR1

Crystal Structure of Lipoprotein GNA1946 from Neisseria meningitidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-03-16
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	109.215, 122.750, 160.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.060 - 2.150
R-factor	0.204
Rwork	0.204
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gxa
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.230
High resolution limit [Å]	2.150	2.150
Number of reflections	108425
Completeness [%]	92.1	51.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	293	2M (NH4)2SO4, 0.1M Citric acid, 0.5% DDM, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K