3IQY
Active site mutants of B. subtilis SecA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 131.015, 131.015, 151.966 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.770 - 3.300 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m6n |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.091 | 0.683 |
| Number of reflections | 22533 | |
| <I/σ(I)> | 22.8 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 299 | 20 mM BES pH 7.0, 2.1 M Ammonium sulfate, 32 % Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 299K |
