3IQW
AMPPNP complex of C. therm. Get3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.570, 105.730, 136.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.860 - 3.000 |
| R-factor | 0.22987 |
| Rwork | 0.228 |
| R-free | 0.27051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ArsA ATPase domain |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.834 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.500 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 14221 | |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.03M MgCl2, 0.1M tris, 33% ethanol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
