3IQT
Structure of the HPT domain of Sensor protein barA from Escherichia coli CFT073.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.316, 60.359, 37.156 |
| Unit cell angles | 90.00, 108.21, 90.00 |
Refinement procedure
| Resolution | 35.300 - 1.400 |
| R-factor | 0.143 |
| Rwork | 0.141 |
| R-free | 0.18100 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.493 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.094 | 0.414 |
| Number of reflections | 19949 | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.05M Calcium Chloride dihydrate, ).1M Bis Tris pH6.5, 30% PEG Monomethyl ether 550, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
