3IQP

SAM-I riboswitch from T. tencongensis variant A94G apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-08-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	62.702, 62.702, 159.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 2.900
R-factor	0.231
Rwork	0.231
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gis
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	CrystalClear
Data scaling software	d*TREK (9.1SSI)
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.910	19.870	2.690
High resolution limit [Å]	2.600	5.560	2.600
Rmerge	0.082	0.028	0.361
Number of reflections	18385
<I/σ(I)>	14.4	41.2	4.4
Completeness [%]	100.0	100	99.8
Redundancy	7.2	7.6	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	7	293	pH 7.0, hanging drop, temperature 293K