3IQP
SAM-I riboswitch from T. tencongensis variant A94G apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.702, 62.702, 159.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 2.900 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gis |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK (9.1SSI) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.910 | 19.870 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.560 | 2.600 |
| Rmerge | 0.082 | 0.028 | 0.361 |
| Number of reflections | 18385 | ||
| <I/σ(I)> | 14.4 | 41.2 | 4.4 |
| Completeness [%] | 100.0 | 100 | 99.8 |
| Redundancy | 7.2 | 7.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 293 | pH 7.0, hanging drop, temperature 293K |
