3IQJ
Crystal Structure of human 14-3-3 sigma in Complex with Raf1 peptide (10mer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87314 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.610, 112.560, 62.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.490 - 1.150 |
| R-factor | 0.12856 |
| Rwork | 0.127 |
| R-free | 0.15267 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ywt |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.140 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.490 | 1.200 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.058 | |
| Total number of observations | 12284 | |
| Number of reflections | 102954 | |
| <I/σ(I)> | 15.2 | 3.8 |
| Completeness [%] | 99.4 | |
| Redundancy | 6.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M Hepes/NaOH ph 7.5, 0.2M CaCl2, 28% PEG 400, 5% glycerol, 2mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
