3IQE
Structure of F420 dependent methylene-tetrahydromethanopterin dehydrogenase in complex with methylene-tetrahydromethanopterin and coenzyme F420
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.148, 165.539, 93.552 |
| Unit cell angles | 90.00, 99.14, 90.00 |
Refinement procedure
| Resolution | 92.450 - 1.800 |
| R-factor | 0.19162 |
| Rwork | 0.190 |
| R-free | 0.22771 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qv9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.627 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.500 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 169214 | |
| <I/σ(I)> | 15.6 | 2.8 |
| Completeness [%] | 95.7 | 94.9 |
| Redundancy | 3.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 30% PEG400, 0.1M MES, 0.1M sodium acetate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
