3IQC
Crystal structure of FliS from H. pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Collection date | 2006-08-28 |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 92.469, 92.469, 144.292 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.140 - 2.700 |
| R-factor | 0.23992 |
| Rwork | 0.238 |
| R-free | 0.28380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1orj |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.922 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.680 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.038 | 0.499 |
| Number of reflections | 8726 | |
| <I/σ(I)> | 24.4 | 3 |
| Completeness [%] | 97.6 | 100 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 289 | 0.1M Hepes, 0.2M NaCl, 25% PEG3350, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
