3IQ0
Crystal structure of a putative Ribokinase II in complex with ATP and Mg+2 from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.304, 87.256, 112.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.630 - 1.790 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 58913 | |
| <I/σ(I)> | 4.9 | 3.8 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 0.2M Magnesium Chloride, 0.1M Bis-Tris 5.5, 25% PEG 3350, 6mM ATP, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
