3IPR
Crystal structure of the Enterococcus faecalis gluconate specific EIIA phosphotransferase system component
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.044 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.680, 69.400, 80.220 |
| Unit cell angles | 90.00, 106.53, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.213 |
| Rwork | 0.189 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pdo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.340 |
| Data scaling software | XSCALE |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 30490 | |
| <I/σ(I)> | 4.42 | |
| Completeness [%] | 94.4 | 94.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 18% PEG 4000, 0.2M Ca acetate, 0.1M Ca cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
