3IPK
Crystal Structure of A3VP1 of AgI/II of Streptococcus mutans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.031, 164.146, 67.733 |
| Unit cell angles | 90.00, 91.03, 90.00 |
Refinement procedure
| Resolution | 42.720 - 2.040 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.22200 |
| Starting model (for MR) | 1jmm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.062 | 0.182 |
| Number of reflections | 66337 | |
| <I/σ(I)> | 29.3 | 8.88 |
| Completeness [%] | 95.9 | 91 |
| Redundancy | 7.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 30% MME PEG 2000, 0.2M Ammonium sulfate, 0.05M Sodium cacodylate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
