3IPI
Crystal Structure of a Geranyltranstransferase from the Methanosarcina mazei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 68.160, 81.273, 49.689 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.460 - 1.900 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25100 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.500 |
| Number of reflections | 22453 | |
| <I/σ(I)> | 12.7 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 14.1 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 2.0 M Sodium Malonate 5% glycerol, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
