3IO0
Crystal structure of EtuB from Clostridium kluyveri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97620 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 173.410, 173.410, 173.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.710 - 3.000 |
| R-factor | 0.18877 |
| Rwork | 0.188 |
| R-free | 0.20337 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.823 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.700 | 3.170 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.151 | 0.640 |
| Number of reflections | 17475 | |
| <I/σ(I)> | 16.8 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 4M formate in water, 8mg/ml protein, equal volumes of reservoir and protein formed the drop, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
